Studies of Lewis antigens and H. pylori adhesion in CHO cell lines engineered to express Lewis b determinants

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Studies of Lewis antigens and H. pylori adhesion in CHO cell lines engineered to express Lewis b determinants.

Many microbes bind and adhere via adhesins to host cell carbohydrates as an initial step for infection. Therefore, cell lines expressing Lewis b (Le(b)) determinants were generated as a potential model system for Helicobacter pylori colonization and infection, and their expression of blood group Lewis determinants was characterized. CHO-K1 cells were stably transfected with selected glycosyltra...

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Expression of Lewis(x), sialylated Lewis(x), Lewis(a), and sialylated Lewis(a) antigens in human lung carcinoma.

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Seven mouse monoclonal antibodies and the lectin Ulex europaeus, detecting blood group related antigens of the ABH and Lewis systems, have been used to determine the immunophenotype of human renal cell carcinomas. Immunohistochemical analyses have demonstrated that these antigenic systems are differentially expressed by distinct normal cell types and domains of the human nephron. In the present...

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ژورنال

عنوان ژورنال: Glycobiology

سال: 2008

ISSN: 0959-6658,1460-2423

DOI: 10.1093/glycob/cwn030